bio blue duo


Acid Blue 90 And Trypan Blue Ophthalmic Solution

BIO-BLUE DUO is a sterile, non-toxic, pyrogen free ready to use solution of Acid Blue 90, Trypan Blue and Polyethylene Glycol. The syringe comes in medical grade blister packaging to ensure safety and protection.


Biotech Bio-Blue Duo is formulated with precise concentrations of its key components:

Trypan Blue (0.15% W/V)

PEG & Acid Blue (Brilliant Blue G) Content (0.025% W/V)

Presentation: The solution is supplied in 0.5 ml pre-filled syringes, equipped with BD Luer-lock Glass Syringe with Cannula for easy and precise administration during surgery.


Biotech Bio-Blue Duo is an innovative ophthalmic solution that revolutionizes vitreoretinal surgery. It offers precise staining capabilities, enabling surgeons to identify and remove specific membranes within the eye with unparalleled accuracy. This advanced solution combines Trypan Blue, Acid Blue 90, and Polyethylene Glycol (PEG) to provide a comprehensive staining solution for various ocular indications. Its unique formulation and features make it a preferred choice for vitreoretinal surgeons seeking optimal outcomes for their patients.

Biotech Bio-Blue Duo is distinguished by several key features that set it apart in the field of ophthalmic surgery:

  • Effective Staining: Biotech Bio-Blue Duo effectively stains internal limiting membranes (ILM), epiretinal membranes (ERM), and proliferative vitreoretinopathy (PVR) membranes, facilitating precise visualization and removal during surgery.
  • Selective Targeting: Biotech Bio-Blue Duo selectively targets the desired tissue, ensuring that only membranes intended for removal are stained. This feature enhances surgical precision and minimizes unnecessary manipulation of healthy tissue.
  • Ready-to-Use: Biotech Bio-Blue Duo is supplied in pre-filled syringes, eliminating the need for additional preparation and ensuring convenience for surgical teams.
  • Compatibility: With the inclusion of Polyethylene Glycol (PEG), Biotech Bio-Blue Duo remains stable and compatible with eyes filled with Balanced Salt Solution (BSS), expanding its applicability in various surgical settings.
  • Controlled Diffusion: Upon injection, Biotech Bio-Blue Duo forms a cohesive ball that travels to the fundus of the eye without diffusing, ensuring targeted staining of the desired tissue.


Biotech Bio-Blue Duo contains a carefully balanced formulation of active ingredients:

  • Trypan Blue: This dye is essential for staining membranes within the eye, enabling surgeons to visualize and differentiate between targeted tissues during surgery.
  • Polyethylene Glycol (PEG): PEG contributes to the stability and compatibility of the solution, allowing for controlled diffusion and targeted staining without compromising surgical outcomes.
  • Acid Blue 90 (Brilliant Blue G): This component enhances the staining properties of Biotech Bio-Blue Duo, ensuring optimal visualization and differentiation of membranes during surgery.


Biotech Bio-Blue Duo is indicated for use in various vitreoretinal surgeries, including but not limited to:

  • Staining the internal limiting membrane (ILM)
  • Staining epiretinal membranes (ERM)
  • Staining proliferative vitreoretinopathy (PVR) membranes

By providing clear differentiation between stained and unstained membranes, Biotech Bio-Blue Duo assists surgeons in identifying and removing specific tissues with precision and ease, thereby improving surgical outcomes and patient recovery.


  • Enhanced Visualization: Biotech Bio-Blue Duo facilitates clear visualization of targeted membranes, allowing surgeons to perform procedures with greater accuracy and confidence.
  • Reduced Surgical Time: The selective staining capability of Biotech Bio-Blue Duo streamlines surgical procedures by minimizing the time required for tissue identification and removal, leading to more efficient surgeries and shorter operative times.
  • Increased Precision: By staining only the desired membranes, Biotech Bio-Blue Duo helps prevent unnecessary manipulation of healthy tissue, reducing the risk of iatrogenic damage and improving overall surgical precision.
  • Improved Patient Outcomes: The precise staining provided by Biotech Bio-Blue Duo contributes to improved surgical outcomes and may lead to faster post-operative recovery times for patients, ultimately enhancing their quality of life.
  • Convenient Presentation: Supplied in pre-filled syringes, Biotech Bio-Blue Duo offers convenience and ease of use for surgical teams, reducing preparation time and minimizing the risk of contamination, thus optimizing workflow efficiency in the operating room.


Stains ILM, ERM as well as PVR membranes effectively.
With PEG, BIO-BLUE DUO can be injected in a BSS filled eye. It travels as a cohesive ball to the fundus of the eye without diffusion and stains the desired tissue selectively.


BIO-BLUE DUO is a single injection designed for staining the membranes, ILM as well as ERM and PVR membrane, without compromising the staining effect. It is injected in the vitreous cavity to clearly differentiate between the stained and unstained part of membranes and helps in easy removal of selected membrane. The combination with PEG 4% is found as stable dye and can also be injected in the eyes filled with BSS. Also it penetrates deep and stains only the targeted membrane or tissue.


Supplied in 0.5 ml PFS, BD Luer-lock Glass Syringe with Cannula